STAGE and PhD



Technobiochip offers to brilliant graduates and PhD students the possibility to enhance their biological, biotechnology and electronic knowledge and interests.


ELECTRONICS

QCM/EQCM quartz oscillator development

For piezoeletric resonant sensor systems the oscillator unit is critical. Technobiochip has carried out many studies and improvements in this field and it is still investing a lot of resources in this research area.

For L1, L2 Stage and Thesis and for PhD students.


QCM/EQCM quartz cell system impedance characterization

For L1 Stage and Thesis.


Capacitive Sensor device (DNA Scan) improvements

The capacitative biosensor developed by Technobiochip consists in a new technological application of a known technology: it correlates, for the first time, the miniaturised capacitative sensor technology to medical and biological methods of analysis, with the aim to develop and produce a new instrument able to reveal the presence of important pathologies or tumorous predispositions.

For L1 and L2 Stage and Thesis.


MOX sensors signal conditioning and acquisition system development

MOX sensors are structures formed by a silicon substrate on which a thin layer of oxide is deposited. The electrical resistance of this structure varies with the type of gas with which it is in contact, with elevated nominal values (10exp6÷10exp9 ohm) and variations from 1 to 3 order

Each sensor also has a resistant element, which is used to heat the system to the required temperature.

The research on the driving electronic architectures and the signals conditioning of this kind of structures is still an open field and Technobiochip is involved in research projects related to the innovation of MOX sensor technology.

For L1 and L2 Stage and Thesis.




BIOLOGY

Development of a QCM biosensor able to reveal the presence of GMO in food.

The present project’s aim is to further develop a QCM biosensor able to reveal the presence of GMO in food, in particular to do real-time monitoring of:

1) The affinity relationship between monoclonal antibodies specific for CP4-synthase and for Cry1A(b) peptides, and the related unmarked antigens.

2) The hybridisation relation between the codified sequences for the two antigens mentioned above and the DNA extracted from the food to be analysed.

For L1 and L2 Stage and Thesis.


The study of a capacitive biosensor to reveal GMO in food.

This project uses the capacitive DNA biosensor developed by Technobiochip for the revelation of GMO in food: in particular, it will monitor the hybridisation reaction between the codified sequences for Cry1A(b ) and the genomic DNA extracted from the food to be analysed.

For L1 and L2 Stage and Thesis


Optimisation of an electrochemical biosensor for the revelation of diagnostic markers of interest.

The electrochemical measurement principles will be based on the use of DNA and specific antibodies, presumably marked with enzymes like phosphatase (acid or alkaline), HRP or glucose oxidase. The aim is to obtain electroactive products which will allow the amperometric revelation in a large range of electrochemical potentials and the possibility to use amplification signal systems.

For L2 Stage and Thesis.


Optimisation of a diagnostic biosensor for tumour screening.

In particular the experimental aim will be in the following fields:

Hybridisation studies for the revelation of tumours DNA, and optimisation of the analytical parameters.

Experiments on diagnostic screening in the case of genic deletions, of genic amplifications and differential allelic amplifications.

Study of the hybridisation using target DNA marked in different ways to obtain a classical control.

Study of the reversibility of the immobilised biomediator to determine the time life of the biosensor.

Selection of the probes, which have given the most sensitive and selective answers.

Analytical optimisation of the obtained results.

For L1 and L2 Stage and Thesis.